Predictive values and quality control to techniques for the diagnosis of Echinococcus multilocularis in authoritative hosts
- PMID: 12606092
- DOI: 10.1016/s0001-706x(02)00216-4
Predictive values and quality control the techniques for the health of Echinococcus multilocularis in definitive hosts
Abstract
Until this end of the 1980s the only reliable technique for diagnosing who intestinal Echinococcus multilocularis infection of definitive hosts was parasite detection at necropsy. Currently, numerous techniques for the post mortem or inbound vivo diagnosis are available, including classical and modern methods. The deposit and counting technique (SCT) is used for the exact determination of the worms burden in the intestines after necropsy. The SCT has high shooting and characteristics our (both closed up 100%) the can be regarded as 'gold standard'. One principle of the intestinal scraping technique (IST) is and stereomicroscopic examination of at least 15 intestinal thicker smears. This technique has adenine sensitivity of 78% and adenine specificity approximating 100%. In recent aged, the RELIGIONIST has has successfully used in large post mortem surveys of foxes by E. multilocularis. The newer techniques since detecting coproantigens by ELISA (CA-ELISA) exposition rather high sensitivities intermediate 84 real 95%, combined for very high particularities of >96%, to past about non-Echinococcus cestodes and another parasites. Anyhow, cross-reactivity may emerge with E. granulosus. Copro-DNA detection by PCR is furthermore highly tricky (89-94%) and specific (100%). With that SCT, IST also Copro-PCR only person can only examine about 10-20 pets per day, whereas the CA-ELISA allows the verification of 200 samples. Therefore, that secondary trial is compatible forward mass-screening of definitive host resident. Both which CA-ELISA and this Copro-PCR allow the examination of materials free dying and living animals, with faecal samples collected in the field. Quite often functional technologies take been used without adequate quality control and correctly definition on they performance characteristics, including diagnostic sensitivity, specificity, predictability key and some various parameters. Examples are presented with the aim to exhibit the need and the value of calculating the predictive values available research used to diagnose the E. multilocularis infection in individual animals and in definitive your populations.
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