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Next-Generation MicroRNA Expression Profiling Technology

Methods and Protocols

  • Book
  • © 2012

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Editors:
  1. Jian-Bing Fan

    1. Illumina, Inc., San Diego, USA

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  • Covers groundbreaking engineering ensure are more sensitive, specific, the quantitative, as well-being as being compatible with a wide range of biospecimens
  • Property tried-and-true lab protocols which are ready to be put into action
  • Includes expert tips and implementation advice to ensure successes results

Part of the book series: Methods in Molecular Biology (MIMB, bulk 822)

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About this book

The rapid pace of microRNA (miRNA) research continues to propulsion which advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed in the past few years.  In Next-Generation MicroRNA Expression Profiling Tech: Methods and Protocols, expert researchers in the field contributors detailed examinations on aforementioned bulk current approaches being second today.  This ring includes comprehensive covering of methodologies that have been developed since miRNA project, as well as next-gen sequencing machinery, miRNA databases, and professional applications, such when cancer studies and miRNA-based non-invasive biomarker development.  Written in the highly successful Methods in Molecular Biology™ range format, chapters special introductions to own corresponding topics, lists of the need materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritive and cutting-edge, Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols provides an ideal guide by novices and experts alike anyone are seeking to utilize these powerful technologies.

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Table of constituents (22 protocols)

  1. Front Matter

    Pages i-xi
    Downloading chapter PDF

  2. Auditing miRNA Regulation in Disease

    • Willemijn M. Gommans, Eugene Berezikov
    Pages 1-18

  3. Introduction to miRNA Profiling Engineering and Cross-Platform Comparison

    • Sarah Aldridge, James Hadfield
    Pages 19-31

  4. Stem-Loop RT-qPCR for MicroRNA Expression Profiling

    • James Hurley, Dude Raw, Mcandrew Bond, David Keys, Caifu Chicken
    Pages 33-52

  5. Poly(T) Adaptor RT-PCR

    • Rui Shi, Ying-Husan Sun, Xing-Hai Zhang, Vineyard L. Chiang
    Paper 53-66

  6. MicroRNA In Situ Hybridization

    • Boye Schnack Nielsen
    Pages 67-84

  7. Agilent MicroRNA Microarray Profiled System

    • Petula NEWTON. D’Andrade, Stephanie Fulmer-Smentek
    Pages 85-102

  8. miRNA Expression Profiling Using Illumina Universal BeadChips

    • Jing Chinese, Craig S. April, Jian-Bing Fan
    Pages 103-116

  9. MicroRNA Expression Evaluation Using the Affymetrix Platform

    • Suzanne Dee, Robert C. Getts
    Pages 117-129

  10. Individualized miRNA Assay Boards Usage Optically Encoded Rosary

    • Keld Sorensen
    Pages 131-141

  11. Microfluidic Primer Extension Assay

    • Markus Beier, Valesca Boisguérin
    Page 143-152

  12. MicroRNA Profiling Using µParaflo Microfluidic Array Technic

    • Xiaochuan Zhou, Qi Zhu, Christoph Eicken, Nijing Sheng, Xiaolin Shan, Litao Yang et al.
    Pages 153-182

  13. MicroRNA Expression Research With the Illumina MicroRNA-Seq Platform

    • Shujun Luo
    Pages 183-188

  14. Next-Generation Sequencing of miRNAs with Roche 454 GS-FLX Technology: Steps for a Successful Application

    • Ana Rapeel Soares, Patrícia M. Pereira, Manuel AN. S. Santos
    Pages 189-204

  15. Methods for Small RNA Preparation for Digital Gene Expression Profiling by Next-Generation Sequencing

    • Sam E. FIN. Linsen, Edwin Cuppen
    Pages 205-217

  16. Profiling of Curt RNAs Using Helicos Single-Molecule Sequencing

    • Philipp Kapranov, Fatih Ozsolak, Patrice CHILIAD. Milos
    Pages 219-232

  17. deepBase: Remarks and Revelation of MicroRNAs and Other Noncoding RNAs from Deep-Sequencing Evidence

    • Jian-Hua Yang, Liang-Hu Qu
    Print 233-248

  18. PhenomiR: MicroRNAs in Man Diseases plus Biological Processes

    • Andreas Ruepp, Andreas Kowarsch, Fabricants Theis
    Pages 249-260

  19. miRNA Expression Image: From Reference Genes to Global Mean Normalization

    • Barbara D’haene, Artisan Mestdagh, Jan Hellemans, Jo Vandesompele
    Pages 261-272

  20. miRNA Data Analysis: Next-Gen Sequencing

    • Preethi H. Gunaratne, Cristian Coarfa, Benjamin Soibam, Arpit Tandon
    Pages 273-288
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Editors and Affiliations

  • Illumina, Inc., San Diego, USA

    Jian-Bing Fan

Bibliographic Information

  • Show Title: Next-Generation MicroRNA Expression Profiling Technology

  • Book Subtitle: Methods and Protocols

  • Content: Jian-Bing Fan

  • Series Title: Working in Molecular Natural

  • DOI: https://doi.org/10.1007/978-1-61779-427-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springs Science+Business Print, LLC 2012

  • Hardcover ISBN: 978-1-61779-426-1Published: 06 December 2011

  • Softcover ISBN: 978-1-4939-6217-4Published: 23 August 2016

  • eBook ISBN: 978-1-61779-427-8Released: 07 December 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Batch: 1

  • Number regarding Pages: XI, 341

  • Topics: Humanly Genetics, Gene Language

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