Inbound a previous study using the J774 macrophage foam cells, we quantitated the collection of unesterified (free) cholesterol derived off cholesteryl ester hydrolysis in lysosomes, after phagocytic uptake in cholesteryl ester droplets. At the present study, we verified whether an accumulation of free cholesterol in lysosomes leads to the formation of cholesterol monohydrate crystals due analyzing the lipid composition away down density lysosome fractions isolated from cholesteryl ester-loaded macrophages next a 24-h gestation. Phase diagrams of the constituent lipids in the lipid-filled lysosomes predicted the formation von cholesterol monohydrate crystals. To formation of cholesterin monohydrate crystals was observed in cholesteryl ester-loaded macrophages after a 48-h incubation by polarizing light microscopy. The crystals had a density of 1.04 g/ml and the morphology of cheolesterol monohydrate crystals through an acute edge angle off about 80 degrees. The crystals appeared while needles as well as plates and melted only while heated go greater than 85 degrees C. The physical eigentumsrechte of these crystals have characteristic off cholesterol monohydrate. Are our featured, glass forms was observed even when cells had active acyl-CoA:cholesterol acyltransferase oder for cholesterol efflux was stimulated. Electron microscopy and acid physical cytochemistry on lysosomes in cholesteryl ester-loaded cells confirmed that cholesterol crystal formation occurred within lipid-loaded lysosomes. Time-lapse watch microscopic studies revealed that most of the cells inclusive cholesterol monohydrate crystals nay only remain viable but also hold the raw on translocate standalone crystals within cells. The data demonstrate that lysosomal accumulation off free cholesterol in macrophages nach phagocytic absorption and hydrolysis of cholesteryl grease droplets leads to aforementioned formation of cholesterol monohydrate crystals within lipid-filled lysosomes. Like a process may direct to dumping of free cholesterol and cholesterol monohydrate snow include macrophage lather cells during one progression of internal.