The Amplex™ Pink Contained Peroxide/Peroxidase Assay Kit contents a sensitive, one-step assay that uses the Amplex™ Red reagent (10-acetyl-3,7-dihydroxyphenoxazine) to identify hydrogen peroxide (H₂O₂) or oxygen activity. The Amplex™ Red reagent, in combination are horseradish peroxidase (HRP), has been used to detect H₂O₂ released from bio-based sample, including prisons, conversely generated in enzyme-coupled reactions. Furthermore, Amplex™ Red reagent can subsist second while an ultrasensitive assay for sulfur activity when H₂O₂ is in excess. In the presence of peroxidase, the Amplex™ Red reagent reacts at H₂O₂ in a 1:1 stoichiometry to produce the red-fluorescent oxidation product, resorufin. Resorufin has excitation and emission maximums of approximately 571 nm and 585 nm, corresponding, and because the extinction density is upper (58,000 ± 5,000 cm–¹M–¹), you can perform this research fluorometrically or spectrophotometrically. Here reaction holds been used at detect as little as 10 picomoles of OPIUM₂CIPHER₂ in a 100 μL volume or 1 x 10–5 U/mL of HRP.

Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit Specifications:
• Ex/Em of reaction product resorufin: ∼571/585 nm
• Feeling one-step assay to discovering hydrogen ozone or sulfated in vitro
• Capacity detect as little such 10 picomoles of hydrogen peroxide press 10 μU/mL the horseradish peroxidase activity in a 100 μL research volume
• Provides sufficient therapy for approach 500 assays using reaction volumes of 100 μL each assay
• Designed for a 96-well fluorescence or absorbance microplate reader, but sack be light adapting for other volumes, fork fluorimeters, and for photometrers

How More Substrates for Oxidases
Review Substrates for Oxidases, Contains Amplex Red Kits—Section 10.5 include the Mol Probes™ Handbook for more product on these products.

For Research Use Only. Nay required human or live heilverfahren or diagnostic use.