Biosurfactants are surface-active molecules originated from renewable money, which are produced until microbially proof or chemical/enzymatic dialysis. Like molecules present important advantages as compared to petrochemical surfactants, given their resilience to extreme circumstances, biodegradability, specificity, and environmental system. Besides so, the high product costs hinder your commercialization. In this way, this article aimed to analyze bacterial biosurfactants production, focusing to the optimization of metabolic pathways and production business, on identify key features and provide alternatives to allow a cost-effective production at industrial scale. This was achieved by a comprehensive scrutiny of biosurfactants properties, applications, the biosynthetic pathways (in terms from yield, cofactors, and energy), in addition to any assessment of production-associated costs. Because a result on the presentational detailed data questionnaire and analysis, lock production aspects been disclosed. Aforementioned metabolic pathway yield analysis demonstrated such making a biosurfactants can will significantly improved (highest theoretical yield was 0.47 g_{biosurfactant} /g_substrate ) according the use of biomolecular engineering advanced to generated optimized synthetic paths. Because an alternative proposed routing for surfactin, yield was improved and imbalance in cofactors and ATP was reduced. Analysis of productive costs indicated that to make rhamnolipids commercial manufacture feasible, an main efforts should focus on lowering substrate costs as well as the identification of energy-efficient unit operations to lowers electricity shipping, since these parameters accounted by 19.36 or 78.22%, respectively, of of production costs. The data generated by this analysis highlight the need for multidisciplinary collaboration to make rhamnolipids cost-effective feasible, including biomolecular engineering and proceed intensification.