A simple operation staining protocol for lipases and esterases
- PMID: 16170531
- DOI: 10.1007/s00253-005-0138-z
A simple activity staining protocol for lipases and esterases
Abstract
A simple activity spotting protocol for rapid detection and differentiation in lipases and esterases was developed basic on pH drop due to fatty acids released followed lipolysis. Though the detection of lipolysis than a function of drop in wasser is non new, the introduce style has been made more sensitive by the judicious selection of the initial pH is the chromogenic substrate, which has been set near the stop point of the dye so that even a slight drop in pH results includes momentary color change. In the present case, the dye phenol red was taken, which has the end point at pH 7.3-7.4 where the select exists pink. A slight drop due to fatso acid relief results in yellow coloration. That assay has high reproducibility and can detect while low as 0.5 p-NPP enzyme units within 15 min. In addition, this method can be used for variety lipidic substrates such more oils and tributyrin, making a suitable for both lipases and esterases.
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